Rebeca Manning Cela PhD

Dra. Rebeca Manning Cela

Cinvestav Researcher 3C

Ph.D. Cellular Biology (1994), Centro de Investigación y de Estudios Avanzados del IPN

Category in the SNI: Level II

Tel: +52 (55) 5061-3800 Ext. 5010
Fax: +52 (55) 5061-3938


Degree: Ph.D. Cellular Biology
Cellular Biology Department, CINVESTAV-IPN

Posdoc: Seattle Biomedical Research Institute. Seattle, WA, USA y Department of Pathobiology University of Washington. Seattle, WA, USA.

Sabbatical: Harvard T.H. Chan School of Public Health, Department of Immunology and Infectious Diseases.

  • Study of the parasite and host cell molecules important for the infection and differentiation process of Trypanosoma cruzi.

  • Motility of T. cruzi and its relationship with its infective capacity.

  • Isolation, characterization, and omics of T. cruzi strains obtained in Mexico.

  • Predictive modeling by georeferencing of the vector, transmission, and risk of Chagas disease.

  • Identification and evaluation of autochthonous T. cruzi antigens, useful for the development of a prototype for the diagnosis of Chagas disease.

Collaborations Institution
Dr. Santiago Martínez Cavillo UBIMED, FES-Iztacala, UNAM
Dra. Ana María Cevallos Gaos Instituto de Biomédicas, UNAM
Dr. Moisés Santillán Zerón CINVESTAV-IPN Unidad Monterrey
Dr. Cesar Israel Lugo Caballero Universidad Autónoma de Yucatán / UADY · Centro de Investigaciones Regionales Dr. Hideyo Noguchi
Dr. Luis Alberto Hernández Osorio Facultad de Ciencias Químicas en la Univ. Autónoma Benito Juárez de Oaxaca
Member and collaboration with the members of the Association of Chagas with Science and Knowledge (ACCyC)  
Dr. Angel Ramos Ligonio Facultad de Ciencias Químicas de la UV campus Orizaba
Dra. Maria del Carmen Guzman Bracho Instituto de Diagnóstico y Referencia Epidemiológicos / InDRE · Secretaría de Salud, Ciudad de México
Dra. Aracely López Monteon Facultad de Ciencias Químicas de la UV campus Orizaba
Dr. Etienne Jean Guy Waleckx Institut de Recherche pour le Développement, UMR INTERTRYP IRD, CIRAD, Université de Montpellier, Montpellier, France / Laboratorio de Parasitología, Centro de Investigaciones Regionales 'Dr. Hideyo Noguchi', Universidad Autónoma deYucatán, Mérida, Yucatán, México
Dr. Victor Manuel Monteón Padilla Centro de Investigaciones Biomédicas, Universidad Autónoma de Campeche
Dra. Maria Elena Villagran Herrera Facultad de Medicina. Universidad Autónoma de Querétaro
Dr. Rubén Acosta Santamaría Director del centro de salud en Sesver

Papers: 40
In extenso papers: 9
Book chapters: 7
Meeting Participation: 140
Graduate students: 18 Bachelor, 16 Master y 10 PhD.
Students in process: 2 Bachelor, 1 Master y 3 PhD
Teaching courses: 58
Coordinated courses: 19

In my laboratory, we are interested in studying the molecular and cellular mechanisms of infectious diseases, particularly in those biological processes that are important to allow Trypanosoma cruzi to efficiently infect humans.

This parasite is the etiological agent of Chagas disease (ChD), a condition that, even when it was discovered more than a century ago, continues to be an incurable, highly disabling disease that generates high treatment costs and very high expenses for the Health sector. It ranks first among tropical diseases and fourth among transmissible diseases, representing a loss of 0.667 million years of disability-adjusted life, which corresponds to the major impact disease in Latin America, and resulting in the more important parasitic infection in endemic countries. In Mexico, a systematic review and meta-analysis of epidemiological studies, estimates an average national seroprevalence of 3.38%, suggesting that there are 4.06 million cases of infection with the parasite, which places it as the country with the highest number of T. cruzi infections in Latin America. This high seroprevalence undoubtedly shows that ChD is an important public health problem in Mexico.

T. cruzi is an obligate intracellular parasite that infects a wide range of cells through a complex infection process that involves the participation of various molecules from both the host cell and the parasite; however, very few molecules have been characterized to date. The infection and differentiation processes are essential for the parasite to complete its life cycle and for its survival. Therefore, one of the lines of research that we are working on is focused on the identification and characterization of the molecules that participate in these processes, using reverse genetic approaches, next generation sequencing analysis and differential omics. In this way, we aim to understand the molecular mechanisms involved in the establishment of the disease and to identify potential targets for the future development of vaccines and drugs, focused on the control of ChD. In this same sense, another line of research in which we work using similar strategies is the characterization of parasite motility and its participation in the infection process of T. cruzi.

The parasite strains comprise six discrete typing units (DTUs) named TcI to TcVI, and a seventh one called TcBat, that circulate among vectors, man, and reservoirs, both domestic and sylvatic cycles, presenting a wide intra-specific variability. This genetic diversity among strains has been related to morphological differences in their blood forms, virulence, ability to induce lesions, susceptibility to chemotherapeutic agents, antigenic constitution, infectivity, geographic distribution, and clinical manifestations of the disease. Therefore, our group has also developed a line of research aimed at the isolation and characterization of autochthonous strains of the parasite used for different purposes.  One of these is the identification and analysis of antigenic proteins and peptides useful for the development of a prototype for the diagnosis of ChD, which would allow the efficient identification of the infection with the parasite strains that circulate in Mexico.

Finally, we carry out epidemiological, entomological, parasitological, and molecular studies, using geographic information systems and spatial modeling for data analysis and establishing correlations that allow the development of spatio-temporal predictive analyses of the behavior of the disease, its transmission and risks. These studies provide useful information that can be used to establish ChD monitoring and control programs.

Year Publication
Florencio-Martínez LE, Cano-Santiago A, Mondragón-Rosas F, Gómez-García M, Flores-Pérez C, Román-Carraro FC, Barocio-Rodríguez LA, Manning-Cela RG, Nepomuceno-Mejía T, Martínez-Calvillo S.
Participation of TFIIIB Subunit Brf1 in Transcription Regulation in the Human Pathogen Leishmania major.
Genes (Basel). 2021 Feb 16;12(2):280.
Jorge A Arias-Del-Angel, Jesús Santana-Solano, Moisés Santillán, Rebeca G Manning-Cela
Motility patterns of Trypanosoma cruzi trypomastigotes correlate with the efficiency of parasite invasion in vitro
Sci Rep. 2020 Sep 28;10(1):15894.
Jorge A Arias-Del-Angel, Rebeca G Manning-Cela, Moisés Santillán
Dynamics of Mammalian Cell Infection by Trypanosoma cruzi trypomastigotes
Front Microbiol. 2020 Oct 2; 11:559660
Margarita Rubio-Ortiz; Laura Alejandra Hernández-López; Anahí Pérez-Galicia, Carmen Guzmán-Bracho; Santiago Martínez-Calvillo; Rebeca Georgina Manning-Cela.
Diagnosis of Trypanosoma cruzi infection: Advances and challenges
Revista Médica de la Universidad Veracruzana Vol. 2020-1: 7-27
Onile, O. S., Awobode H. O., Agunloye A. M., Marquez-Duenas C., Manning Cela R. G. and Anumudu C. I.
IL4, IL13, GSTM1 and T1 variants and susceptibility to Schistosomiasis and associated bladder pathologies in Eggua, Nigeria.
Nig. J. Biotech. Vol. 37(1): 58-72 (June 2020)
González-Chávez Z, Vázquez C, Mejia-Tlachi M, Márquez-Dueñas C, Manning-Cela R, Encalada R, Rodríguez-Enríquez S, Michels PAM, Moreno-Sánchez R, Saavedra E.
Gamma-glutamylcysteine synthetase and tryparedoxin 1 exert high control on the antioxidant system in Trypanosoma cruzi contributing to drug resistance and infectivity.
Redox Biol. 2019 May 28;26:101231.
Román-Carraro FC, Florencio-Martínez LE, Romero-Meza G, Nepomuceno-Mejía T, Carrero JC, Arroyo R, Ortega-López J, Manning-Cela RG, Martínez-Calvillo S.
TFIIIB Subunit Bdp1 Participates in RNA Polymerase III Transcription in the Protozoan Parasite Leishmania major
Biomed Res Int. 2019 Apr 1;2019:1425281.
Vizcaíno-Castillo A, Osorio-Méndez JF, Rubio-Ortiz M, Manning-Cela RG, Hernández R, Cevallos AM.
Trypanosoma cruzi actins: Expression analysis of actin 2
Biochem Biophys Res Commun. 2019 May 28;513(2):347-353.
Martínez-Calvillo S, Romero-Meza G, Vizuet-de-Rueda JC, Florencio-Martínez LE, Manning-Cela R, Nepomuceno-Mejía T.
Epigenetic Regulation of Transcription in Trypanosomatid Protozoa.
Curr Genomics. 2018 Feb;19(2):140-149.
Pardavé-Alejandre HD, Alvarado-Yaah JE, Pompa-Mera EN, Muñoz-Medina JE, Sárquiz-Martínez B, Santacruz-Tinoco CE, Manning-Cela RG, Ortíz-Navarrete V, López-Macías C, González-Bonilla CR.
Autodisplay of an avidin with biotin-binding activity on the surface of Escherichia coli.
Biotechnol Lett. 2018 Mar;40(3):591-600.
Romero-Meza G, Vélez-Ramírez DE, Florencio-Martínez LE, Román-Carraro FC, Manning-Cela R, Hernández-Rivas R, Martínez-Calvillo S.
Maf1 is a negative regulator of transcription in Trypanosoma brucei.
Mol Microbiol. 2017 Feb;103(3):452-468.
Osorio-Méndez JF, Vizcaíno-Castillo A, Manning-Cela R, Hernandez R, Cevallos AM.
Expression of profilin in Trypanosoma cruzi and identification of some of its ligands.
Biochem Biophys Res Commun. 2016 Nov 25;480(4):709-714.
Moreno-Campos R, Florencio-Martínez LE, Nepomuceno-Mejía T, Rojas-Sánchez S, Vélez-Ramírez DE, Padilla-Mejía NE, Figueroa-Angulo E, Manning-Cela R, Martínez-Calvillo S.
Molecular characterization of 5S ribosomal RNA genes and transcripts in the protozoan parasite Leishmania major.
Parasitology. 2016 Dec;143(14):1917-1929.
Rojas-Sánchez S, Figueroa-Angulo E, Moreno-Campos R, Florencio-Martínez LE, Manning-Cela RG, Martínez-Calvillo S.
Transcription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene.
Parasit Vectors. 2016 Jul 19;9(1):401.
Vizuet-de-Rueda JC, Florencio-Martínez LE, Padilla-Mejía NE, Manning-Cela R, Hernández-Rivas R, Martínez-Calvillo S.
Ribosomal RNA Genes in the Protozoan Parasite Leishmania major Possess a Nucleosomal Structure.
Protist. 2016 Apr;167(2):121-35.
Sosa-Hernández E, Ballesteros-Rodea G, Arias-Del-Angel JA, Dévora-Canales D, Manning-Cela RG, Santana-Solano J, Santillán M.
Experimental and Mathematical-Modeling Characterization of Trypanosoma cruzi Epimastigote Motility.
PLoS One. 2015 Nov 6;10(11):e0142478.
Vélez-Ramírez DE, Florencio-Martínez LE, Romero-Meza G, Rojas-Sánchez S, Moreno-Campos R, Arroyo R, Ortega-López J, Manning-Cela R, Martínez-Calvillo S.
BRF1, a subunit of RNA polymerase III transcription factor TFIIIB, is essential for cell growth of Trypanosoma brucei.
Parasitology. 2015 Nov;142(13):1563-73.
Padilla-Mejía NE, Florencio-Martínez LE, Moreno-Campos R, Vizuet-de-Rueda JC, Cevallos AM, Hernández-Rivas R, Manning-Cela R, Martínez-Calvillo S.
The selenocysteine tRNA gene in leishmania major is transcribed by both RNA polymerase II and RNA polymerase III.
Eukaryot Cell. 2015 Mar;14(3):216-27.
Angulo-Rojo C, Manning-Cela R, Aguirre A, Ortega A, López-Bayghen E.
Involvement of the Notch pathway in terminal astrocytic differentiation: role of PKA.
ASN Neuro. 2013 Dec 23;5(5): 363-376.
Lugo-Caballero C., Ballesteros-Rodea G., Martínez-Calvillo S., Manning-Cela R.
Identification of protein complex associated with LYT1 of Trypanosoma cruzi.
BioMed Research International (Antes Journal of Biomedicine and Biotechnology)
Biomed Res Int. 2013;2013:493525.
Gilberto Ballesteros-Rodea, Moisés Santillán, Marisa Cruz-Aguilar, Claudia Márquez-Dueñas, César Israel Lugo-Caballero, Santiago Martínez-Calvillo, John Swindle y Rebeca Georgina Manning-Cela.
The alternative products of Trypanosoma cruzi LYT1 have different localization patterns.
Vet. Mex (2012): 43 (1): 29-43.
Ballesteros-Rodea, G., Santillán M., Martínez-Calvillo S., and Manning-Cela R.
Flagellar Motility of Trypanosoma cruzi Epimastigotes.
Journal of Biomedicine and Biotechnology J Biomed Biotechnol. 2012;2012:520380.
doi: 10.1155/2012/520380.
Erratum in: J Biomed Biotechnol. 2012;2012:793487.
Volume 2012, Article ID 520380, 9 pages.
Cevallos AM, Segura-Kato YX, Merchant-Larios H, Manning-Cela R, Hernández-Osorio LA, Márquez-Dueñas C, Ambrosio JR, Reynoso-Ducoing O, Hernández R.
Trypanosoma cruzi: Multiple actin isovariants are observed along different developmental stages.
Experimental Parasitology 2011 Jan;127(1):249-59.
Luis Florencio-Martínez, Claudia Márquez-Dueñas, Gilberto Ballesteros-Rodea, Santiago Martínez-Calvillo, Rebeca Manning-Cela.
Cellular analysis of host cell infection by different developmental stages of Trypanosoma cruzi.
Experimental Parasitology (2010): 10 Nov;126(3):332-6.
Santiago Martínez-Calvillo, Juan C. Vizuet-de-Rueda, Luis E. Florencio-Martínez, Rebeca G. Manning-Cela and Elisa E. Figueroa-Angulo
Gene expression in trypanosomatid parasites.
Journal of Biomedicine and Biotechnology. 2010;2010:525241.
Hernández-Osorio LA, Márquez-Dueñas C, Florencio-Martínez LE, Ballesteros-Rodea G, Martinez-Calvillo S, and Manning-Cela RG.
Improved Method for in vitro Secondary Amastigogenesis of Trypanosoma cruzi: Morphometrical and Molecular Analysis of Intermediate Developmental Forms
Journal of Biomedicine and Biotechnology, 2010; 2010:283842.
Padilla-Mejía NE, Florencio-Martínez LE, Figueroa-Angulo EE, Manning-Cela RG, Hernández-Rivas R, Myler PJ, Martínez-Calvillo S.
Gene organization and sequence analyses of transfer RNA genes in Trypanosomatid parasites.
BMC Genomics. 2009 May 18;10:232.
Peralta J, Manuel Hernandez JM, Manning-Cela R, Luna-Muñoz J, Garcia-Tovar C, Nougayréde JP, Oswald E and Navarro-Garcia F.
EspF interacts with nucleation-promoting factors to recruit junctional proteins into the pedestals for their maturation and disruption of the paracellular permeability.
Infect Immun. 2008 Sep;76(9):3854-68.
Manning-Cela R, Jaishankar S, and Swindle S.
Life-cycle and growth phase dependent regulation of the ubiquitin genes of Trypanosoma cruzi.
Arch Med Res. 2006 Jul ;37 (5): 593-601.
Bautista R, Manning R, Martinez F, Del Carmen Avila-Casado M, Soto V, Medina A, and Escalante B.
Angiotensin II-dependent increased expression of Na+-glucose cotransporter in hypertension.
Am J Physiol Renal Physiol. 2004 Jan;286(1):F127-33.
Manning-Cela R, Marquez C, Franco E, Talamas-Rohana P, and Meza I.
BFA-sensitive and insensitive exocytic pathways in Entamoeba histolytica trophozoites: their relationship to pathogenesis.
Cell Microbiol. 2003 Dec;5(12):921-932.
Manning-Cela, R., Gonzalez, A., and Swindle, J.
Alternative Trans-splicing of the LYT1 Gene of Trypanosoma cruzi.
Infect Immun. 2002 Aug;70(8):4726-8.
Franco E, Manning-Cela R, And Meza I.
Signal transduction in Entamoeba histolytica Induced by Interaction with Fibronectin: Presence and Activation of PKA and Its Posible Relation to Invasiveness.
Arch Med Res. 2002 Jul-Aug;33(4):389-397.
Manning-Cela R, Cortes A, Gonzalez-Rey E, Van Voorhis WC, Swindle J, Gonzalez A.
LYT1 protein is required for efficient in vitro infection by Trypanosoma cruzi.
Infect Immun. 2001 Jun;69(6):3916-23.
Franco, E., Manning-Cela, R. and Meza, I.
Signal Transduction Pathways in Entamoeba histolytica: PKA Activity and Translocation During the Interaction of Trophozoites with FN.
Arch Med Res; 31:S126-S127.
Manning-Cela, R., Carbaja,l E. and Meza, I.
Secretory Pathway Activation by Interaction of Entamoeba histolytica Trophozoites with Fibronectin.
Manning-Cela, R., Piña, A., and Meza, I. 1997.
CAMP levels and up-regulation of actin mRNA in Entamoeba histolytica.
Arch Med Res; 28:134-135.
Manning-Cela, R. and Meza, I. 1997.
Up-regulation of actin mRNA and reorganization of the cytoskeleton in Entamoeba histolytica trophozoites.
The Journal of Eukaryotic Microbiology, 44:18-24.
Carbajal, M.E., Manning-Cela, R., Pina, A. and Meza, I. 1996.
Fibronectin-Induced Intracellular Calcium Rise in Entamoeba histolytica Trophozoites: Effect on Adhesion and the Actin Cytoskeleton.
Experimental Parasitology, 82: 11-20.
Cázares, F., Manning-Cela, R. and Meza, I. 1994.
Heterogeneity of the Ribosomal DNA Episome in Strain and Species of Entamoeba.
Molecular Microbiology;12(4):607-612.
Manning-Cela, R., Meraz, M.A., Hernandez J.M. and Meza, I. 1994.
Actin mRNA Levels and Actin Synthesis During the Encystation of Entamoeba invadens. The Journal of Eukaryotic Microbiology; 41(4):360-365.
Capítulos de Libro  
Lugo-Caballero C, Sánchez-Cruz D, Ballesteros-Rodea G, Hernández-Osorio, Noris-Saravia, Martínez-Calvillo y Manning-Cela
Título del Capitulo: Trypanosomiasis Americana
En: Parasitosis más comunes en la población mexicana.
Editores: Jorge Morales Motor, Abraham Landa Piedra, Luis Ignacio Terrazas y Romel Hernández Bello.
Universidad Autónoma de Nuevo León
Casa del Libro. Padre Mier 909 poniente, esquina con Vallarta Centro, Monterrey, Nuevo León, México,
ISBN 978-607-27-0110-6
Santiago Martínez-Calvillo, Rodrigo Moreno-Campos, Gabriela Romero-Meza, Saúl Rojas-Sánchez, Daniel E. Vélez-Ramírez, Rebeca G. Manning-Cela y Luis E. Florencio-Martínez
Título del capítulo: Leishmaniasis
En: Parasitosis más comunes en la población mexicana.
Editores: Jorge Morales Motor, Abraham Landa Piedra, Luis Ignacio Terrazas y Romel Hernández Bello.
Universidad Autónoma de Nuevo León
Casa del Libro. Padre Mier 909 poniente, esquina con Vallarta Centro, Monterrey, Nuevo León, México,
ISBN 978-607-27-0110-6
C. Lugo-Caballero, D. Sánchez-Cruz, L.A. Hernández-Osorio, G. Noris-Saravia, M. Rubio-Ortíz, C. Marquez-Dueñas, S. Martínez-Calvillo and R. Manning-Cela.
Chapter Title: Trypanosoma cruzi genome: organization, dynamics, function and promise.
In: Comparative Genomics in Neglected Human Parasites
César López-Camarillo and Laurence A. Marchant Editors.
Published by Nova Science Publishers,, Inc. New York. 2013
ISBN: 978-1-62417-874-0
Norma E. Padilla-Mejía, Claudia M. Gómez-Hurtado, Inti I. Sánchez-Santamaría, Luis E. Florencio-Martínez, Rebeca G. Manning-Cela and Santiago Martínez-Calvillo.
Chapter Title: Comparative Genomics of Leishmania parasites.
In: Comparative Genomics in Neglected Human Parasites
César López-Camarillo and Laurence A. Marchant Editors.
Published by Nova Science Publishers,, Inc. New York. 2013
ISBN: 978-1-62417-874-0
Santiago Martínez Calvillo, Luis E. Florencio Martínez, Rebeca G. Manning Cela y Elisa E. Figueroa Angulo.
Estructura genómica y regulación de la expresión genética en Leishmania major y Trypanosoma cruzi.
Genómica de Parásitos: Aspectos Moleculares, Genómicos, Proteómicos y de Virulencia.
ISBN: 978-3-8484-7723-4
Editorial Académica Española. LAP LAMBERT Academic Publishing GmbH & Co. KG. Printed in the U. K.
Editor: María Elizbeth Alvarez Sánchez, Elisa Azuara 2012
Bertha Espinoza Gutierrez and Rebeca G. Manning-Cela.
An overview of mammalian cell infection by Trypanosoma cruzi: Cellular and molecular basis.
Advances in the immunobiology of parasitic disases 2007.
ISBN: 81-308-0166-3
Editor: Luis I. Terrazas
Research Signpost.
Kerala, India. 2007
Manning-Cela R.
Papel de LYT1 en el Proceso de Infección de Trypanosoma cruzi.
En Diagnóstico de Enfermedades Infecciosas Emergentes.
ISBN 970-94592-0-1
Ed. Laboratorios Silanes SA de CV., Sociedad Mexicana de Inmunología AC, Consejo Nacional de Ciencia y Tecnología y Facultad de Medicina de la UNAM, México. Páginas 93-102.
Editores: Jorge F. Paniagua Solís, Leopoldo Santos Argumedo y Vianney Ortiz Navarrete, 2005
Artículos de difusión  
Margarita Rubio-Ortiz, Gabriel Noris-Sarabia, Santiago Martínez-Calvillo y Rebeca Georgina Manning-Cela
Chagas, una enfermedad olvidada: descubrimiento único en la medicina tropical y un problema actual de salud global
Avance y Perspectiva, Volumen 5, Número 2, pg. 1-6, 2019
Alberto Antonio-Campos, Teresa I. Martínez-Cuevas, Luis Alberto Hernández-Osorio, Santiago Martínez-Calvillo y Rebeca Georgina Manning Cela
Enfermedad de Chagas: Vectores
Revista de la Academia Mexicana de Ciencias 2017
Volumen 68 número 1:30-33
Margarita Rubio-Ortíz, Gabriel Noris-Sarabia, Santiago Martínez-Calvillo y Rebeca Georgina Manning-Cela
Biología molecular de protozoarios parásitos
Revista de la Academia Mexicana de Ciencias 2017
Volumen 68 número 1:10-13
Cargo Nombre Correo electrónico
Auxiliar de investigación Q.F.B. Claudia Marquez Dueñas
Estudiante de Doctorado M.G. Mónica Chico Avelino
Estudiante de Doctorado M.C. Anahi Pérez Galicia