Vianney Ortiz Navarrete PhD

Vianney Ortiz-Navarrete Ph.D.
Professor 3C
National Research III
Phone: (55) 5747 3324
Laboratory phone: (55) 5747 3800 ext 5009
vortiz@cinvestav.mx

 

Our laboratory has focused on studying various aspects of the innate and adaptive immune response. We look at the host-parasite relationship between mouse B-lymphocytes and Salmonella typhimurium. We aim to understand bacteria's mechanisms to turn B-lymphocytes into their ideal niche to survive prolonged periods without affecting the B lymphocytes' viability. And becoming a persistent bacterium within them and whether this chronic infection could impact the generation of lymphoma and/or leukemia.

On the other hand, we characterize structurally and functionally the CRTAM molecule (Class-I Restricted T cell-Associated Molecule). This protein, also known as CD355, is expressed in activated T cells, NKT lymphocytes, and NK cells. It is also expressed in neutrophils and eosinophils from patients with allergic asthma.  CD355 is essential for CD8 T lymphocytes, NKT, and NK cells to efficiently carry out their cytotoxic and/or IFN-gamma production function. We focused on understanding how its structure participates in the interaction with its ligand, Nectin-like and how it impacts the signaling pathways that CD355 uses to carry out its functions. We are also involved in producing monoclonal antibodies that block the interaction ligand-receptor to stop IFN production.

Additionally, we studied the neutrophils that produce IL-17. Our findings indicate that the frequency of this subpopulation increase in patients with allergic asthma and in patients with rheumatoid arthritis. Therefore, we are interested in identifying the molecules that participate in the activation of these neutrophils and the mechanisms that give them the capacity to produce IL-17. To identify targets to block the production of this cytokine during chronic inflammation.

Methods commonly employed in the laboratory

  • Culture of cell lines
  • Cultivation of bacteria (Salmonella)
  • Tinsiones of direct and indirect immunofluorescence evaluated by flow cytometry and confocal microscopy
  • PCR and qPCR
  • Electrophoresis of proteins and DNA
  • Western blot
  • ELISA
  • Gene cloning
  • Transfection of cells
  • Lymphocyte isolation and purification from organs of mice or human peripheral blood

  • Abraham García-Gil, Carlos-Samuel Galán-Enríquez, Araceli Pérez-López, Porfirio Nava, Celia Alpuche-Aranda, Vianney Ortiz-Navarrete.. Salmonella effector SopB promotes the Akt-YAP pathway activation to promote its survival in B cells. 2018. Virulence19; 1390-1402. doi: 10.1080/21505594.2018.1509664.

  • Serrano C, Galán S, Rubio JF, Candelario-Martínez A, Montes-Gómez AE, Chánez-Paredes S, Cedillo-Barrón L, Schnoor M, Meraz-Ríos MA, Villegas-Sepúlveda N, Ortiz-Navarrete V and Nava P. 2019 Compartmentalized Response of IL-6/STAT3 Signaling in the Colonic Mucosa Mediates Colitis Development. J Immunol 202 (4):1239-1249.

  • Garcia-Gil A, Lopez-Bailon LU and Ortiz-Navarrete V. 2019. Beyond the antibody: B cells as a target for bacterial infection. J Leuk Biol 105 (5): 905-913. Jan 18. doi: 10.1002/JLB.MR0618-225R.

  • Ramirez-Ramirez D, Padilla-Castañeda S, Galan-Enriquez CS, Vadillo E, Prieto-chavez JL, Jimenez-Hernandez E, vilchis-Ordoñez A, Sandoval A, Balandran JC, Perez-Tapia SM, Ortiz-Nvarrete V, Pelayo-Camacho R. 2019.CRTAM+NKcells endowed with suppressor properties arise in leuykemic bone marrow. J Leukoc Biol 105 (5):999-1013. doi: 10.1002/JLB.MA0618-231R]

  • Maria Gonzalez-Orozco, Rosa E Barbosa-Cobos, Lizbeth Becerril-Mendoza, Leonardo Limon-Camacho, Ana I Juarez-Estrada, Gustavo E. Lugo-Zamudio, Jose Moreno-Rodriguez and Vianney Ortiz-Navarrete. 2019. Endogenous stimulation is responsible for the high frequency of IL-17A-producing neutrophils in patients with rheumatoid arthritis. Allergy, Asthma Clin Immunol, 15:44, doi: 10.1186/s13223-019-0359-9. eCollection 2019.

  • Juan Carlos Barragan-Galvez,· Maria Gonzalez-Orozco, Araceli Hernandez-Flores, Jose Luis Maravillas-Montero, Yedhani Chavez-Guerrero, Vianney Ortiz-Navarrete. 2020. Prokaryotic Expression of the Immunoglobulin’s Domains of CRTAM to Characterize a Monoclonal Antibody. Protein J. 39:224-231.

  • Julio Angel Vázquez-Martínez, Miguel Angel Gómez-Lim, Edgar Morales-Ríos, Jorge Alberto Gonzalez-y-Merchand and Vianney Ortiz-Navarrete. 2020 Short-disordered epitope of CRTAM Ig-like V domain as a potential target for blocking antibodies. Int J Mol Sci. 2020;21(22):8798. doi: 10.3390/ijms21228798

  • Sosa-Hernández VA, Torres-Ruíz J, Cervantes-Díaz R, Romero-Ramírez S, Páez-Franco JC, Meza-Sánchez DE, Juárez-Vega G, Pérez-Fragoso A, Ortiz-Navarrete V, Ponce-de-León A, Llorente L, Berrón-Ruiz L, Mejía-Domínguez NR, Gómez-Martín D, Maravillas-Montero JL. 2020. B Cell Subsets as Severity-Associated Signatures in COVID-19 Patients.. Front Immunol;11:611004. doi: 10.3389/fimmu.2020.611004. eCollection 2020.

  • Israel Parra-Ortega, Noemí Nájera-Martínez, Félix Gaytán-Morales, Iván Castorena-Villa, Catalina Cortés-Flores, Briceida López-Martínez, Vianney Ortiz-Navarrete, and Irlanda Olvera-Gómez. 2020. Natural killer cell reconstitution after hematopoietic stem-cell transplantation in children. Gaceta de Mexico, 156:188-194

  • Roberto Rosales-Reyes, Paola Garza-Villafuerte, Daniela Vences-Vences, Daniel F. Aubert, Rubi Aca-Teutle, Vianney F. Ortiz-Navarrete, Laura C. Bonifaz, Julio Cesar Carrero-Sánchez, Alfonso Olivos-García, Miguel A. Valvano, and José Ignacio Santos-Preciado. 2020. Interferon-gamma-activated macrophages infected with burkholderia cenocepacia process and present bacterial antigens to t-cells by class Iand II major histocompatibility complex molecules. Emerging Microbes & Infections, 9:2000–2012.

  • Esther López-Bayghen, Karla Pérez-Toledo,Leticia Ramírez-Martínez, Jacqueline Robledo, Vianney Ortiz and Arturo Ortega. 2020. Class-I MHC-Restricted T-Cell. Associated Molecule (CRTAM) Expression in Cerebellum. Acta Scientific Neurology 3:3-11.

  • Sanyog Dwivedi, Erika P. Rendón-Huerta, Vianney Ortiz Navarrete, Luis F. Montaño. 2021. CD38 and regulation of the immune response cells in cancer. J Oncology. Volume 2021 |Article ID 6630295

Dr. Vianney Ortiz Navarrete
Biochemist, University of Veracruz, Mexico.
MS and Ph.D. -Immunology, National Polytechnic, Mexico (IPN).
Post-Doctoral German Cancer Research Center. Heidelberg, Germany.

Lab Manager: Biol. Benjamín Ortiz López
Biology, National Autonomous University of Mexico (UNAM)
Research Technician: Jesús Guzmán Campuzano
Ph.D. Students
  

Gabriela Hernández Galicia
Biochemist., University of Tabasco, Mexico.
MS- Molecular Biomedicine, Cinvestav, Mexico.
Ph.D. Project: Role of CRTM in the generation of memory CD8 T cells.
gabriela.hernandez@cinvestav.mx

Laura Denise Manzanares Meza
Biochemist., University of Durango, Mexico.
MS- Molecular Biomedicine, Cinvestav, Mexico.
Ph.D. Project: Dissenting macrophage´s mechanisms for IL-36? secretion
Co-Tutor: Dr. Oscar Medina Contreras (Hospital Infantil de México Federico Gómez)
denise.manzanares@cinvestav.mx

Sanyog Dwivedi
Bachelor degree Biotechology Shobhit University. India.
Master of Technology Biotechnology. Amity University Noida. India
Proyecto de Doctorado en BM: Effect of Claudin 6 and Claudin 9 on NK cell cytotoxic activity against AGS cell line.
Co-tutor: Dr. Luis Felipe Montaño (Facultad de Medicina, UNAM)
sanyong.sanyong@cinvestav.mx

Víctor Andrés Sosa Hernández
Medical Doctor, National Polytechnic (IPN), Mexico
MS-Molecular Biomedicine, Cinvestav, Mexico
Ph.D. project: B cell response of Covid-19 patients
Co-tutor: Dr. José Luis Maravillas Montero (Red de Apoyo a la Investigación, Universidad Nacional Autónoma de México e Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán)
victor.sosah@cinvestav.mx

Uriel Juárez Hernández
Medical Doctor, National Autonomous University of Mexico (UNAM).
MS-Health Science, National Polytechnic (IPN).
Ph.D project: Anti-tumor activity of peptides from BH3 domain of Bax secreted by an attenuate Salmonella enterica Serovar Typhimurium
Co-turor: Dr. Rosendo Luria Perez (Hospital Infantil de México Federico Gómez).
uriel.juarez@cinvestav.mx

Luis Uriel López Bailón
Biochemist, National Polithenic (IPN), Mexico.
MS-immunology, National Polithenic (IPN), Mexico.
Ph.D. project: Role of autophagy during Salmonella infection of B lymphocytes.
Co-tutora: Dra. Martha Moreno Lafont (ENCB, IPN)
llopezb1002@alumno.ipn.mx

Luis Gómez Morales
Medical Doctor, Military School of Medicine, Mexico
MS-Immunology, Military School of Medicine, Mexico
Ph.D project: Characterization of IL17- producing neutrophils from patients with rheumatoid arthritis.
lugo0570@colorado.edu
Master in Science Students
  

Diana Berenice Ríos Ramírez
Biochemist, National Autonomous University of Mexico (UNAM)
MS project: identification and characterization of the pathway signaling induced by TGF-ß on Treg exposed to tumor microenvironment.
Co-tutora: Dra. Paula Licona Limón (Instituto de Fisiología Celular, UNAM).
diana.rios@cinvestav.mx

Ana Daniela González Telona
Biologist, National Autonomous University of Mexico (UNAM).
MS project: Mechanism involves in generation of persistent Salmonella within infected B-cells.
ana.gonzalezt@cinvestav.mx